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Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Bovine DNA detection method

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Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR - Bovine DNA detection method1 Scope This document specifies a real time polymerase chain reaction (real time PCR) method for the qualitative detection of bovine specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of bovine material derived from cattle including taurine (Bos taurus) and zebu (Bos indicus). The assay also detects the species bison (Bison bison)

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