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Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Requirements for sample preparation for qualitative detection

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Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Requirements for sample preparation for qualitative detection1 Scope This International Standard provides criteria and examples for sample preparation in order to obtain PCR compatible samples or nucleic acids of suitable quality and quantity for PCR. It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B. This International Standard has been established

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